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Hello everyone,
I'm trying to automate some data collection, and I need a consensus sequence generator.
Thus far, I've been using Geneious's built in consensus sequence generator, which has worked well. The only issue is that it is not command line accessible.
I found that the samtools mpileup command shown at http://samtools.sourceforge.net/mpileup.shtml generates a consensus sequence of the same length as the Geneious consensus but is only ~93% identical.
What might be causing these differences? Does anyone else know of a good standalone consensus sequence generator?
Thanks
I'm trying to automate some data collection, and I need a consensus sequence generator.
Thus far, I've been using Geneious's built in consensus sequence generator, which has worked well. The only issue is that it is not command line accessible.
I found that the samtools mpileup command shown at http://samtools.sourceforge.net/mpileup.shtml generates a consensus sequence of the same length as the Geneious consensus but is only ~93% identical.
What might be causing these differences? Does anyone else know of a good standalone consensus sequence generator?
Thanks