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Do you guys have any idea which NGS platform is the most suitable for doing the whole genome rearrangement project ?
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Bioinformatics Intern.
Master program in Bioinformatics. -
Any paired-end or long read platform will be adequate.
Solexa, SOLiD and 454 will all work for this type of project, and you should consider how much you're planning to sequence and what resources are available to you to decide which to use.Mendelian Disorder: A blogshare of random useful information for general public consumption. [Blog]
Breakway: A Program to Identify Structural Variations in Genomic Data [Website] [Forum Post]
Projects: U87MG whole genome sequence [Website] [Paper] -
Also, if you're looking at structural variation ... a mate-paired library would be ideal.Nicolas Tremblay
Graduate Student
Cardiovascular Genetics - Andelfinger Lab
CHU Ste-Justine Research CenterComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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