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Hello,
I have resequenced 54 closely-related strains of yeast and now I would like to assemble them into a phylogenetic tree just to make sure they are related as I expect them to be. I'm new to next gen sequencing so this serves as a check for my sequence alignment and assembly methods as well
However, I can't find any programs that can handle such a large task. The most impressive software I can find tends to be built to incorporate many taxa, not longer sequences (mine are each 12.5Mb long). Does anyone have any suggestions of how to do this?
Thank you!
Roxana
I have resequenced 54 closely-related strains of yeast and now I would like to assemble them into a phylogenetic tree just to make sure they are related as I expect them to be. I'm new to next gen sequencing so this serves as a check for my sequence alignment and assembly methods as well
However, I can't find any programs that can handle such a large task. The most impressive software I can find tends to be built to incorporate many taxa, not longer sequences (mine are each 12.5Mb long). Does anyone have any suggestions of how to do this?
Thank you!
Roxana