Hello,
I'm searching for a tool for to get corrected gene sequence by resequencing.
I have some data from RNA-seq. Since the genome organism is not
available, I mapped them to reference genes of related species.
From such result, it will be easy to obtain the cDNA sequences of the target
organism, if there are only mismatches due to substitution.
However, when there are small indels, to correct the reference sequence
using mapped data such as SAM data seems not so simple.
Could you tell me about some convenient tools or methods to correct the reference gene sequences using mapping data?
Thank you in advance.
Naoaki ONO
I'm searching for a tool for to get corrected gene sequence by resequencing.
I have some data from RNA-seq. Since the genome organism is not
available, I mapped them to reference genes of related species.
From such result, it will be easy to obtain the cDNA sequences of the target
organism, if there are only mismatches due to substitution.
However, when there are small indels, to correct the reference sequence
using mapped data such as SAM data seems not so simple.
Could you tell me about some convenient tools or methods to correct the reference gene sequences using mapping data?
Thank you in advance.
Naoaki ONO
