I am interested in sequencing a small fragment of the MHC and am researching Next Generation techniques that promise to reduce error. The standard protocol for sequencing these genes involved a tremendous amount of cloning and Sanger sequencing.
Does anyone have any experience conducting Duplex sequencing as described by the article below?
This technique was developed to look for very rare mutations, but it could also be used to find MHC alleles which are known to have multiple repeats in an individual. Any thoughts?
Does anyone have any experience conducting Duplex sequencing as described by the article below?
This technique was developed to look for very rare mutations, but it could also be used to find MHC alleles which are known to have multiple repeats in an individual. Any thoughts?
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