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Does anyone have a protocol for paired-end for Agilent's SureSelect array-based sequence capture?
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Do you have an issue with Agilent's protocol? -
The only Agilent protocol I have is for single-end and I'm interested in trying paired-end for the Illumina GA.Comment
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Version 1.0, September 2009
(top hit [.pdf])Comment
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Thanks but I'm specifically looking for paired-end for the array-based capture. While Agilent does have protocols for paired-end in-solution capture products (I've used those and they work quite well), Agilent only offers a protocol for single-end for the array-based capture product.Comment
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Oh. Any particular reason you're going with array based then, if solution based works so well?Comment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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