Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • chariko
    Member
    • Jun 2010
    • 56

    #1

    Negative start position bed file

    I aligned two samples (bwa; filter with samtools, removed duplicates with picard-tools, bamtobed and bed to bedgraph with genomeCoverageBed) to a genome file consisting in 4 genes and the first sample went fine but the second one when getting the bed file the start position for one of the genes was -1. I wonder that is not correct but I do not know why did I obtain such negative value.

    Example:

    "Wrong bed file for gene1"
    gene1 -1 150 M00... 37 -
    gene1 -1 150 M01... 37 +
    gene2 1004 1115 M00... 60 -

    I realized that -1 values in special cases, however, BEDPE (bed paired end) positions may be set to -1 to indicate that one or more ends of a BEDPE feature is unaligned.



    but I do not understand if that´s my case and I really do not understand why did it align if it was unaligned....

    Any clues?

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 11:13 AM
0 responses
16 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, Yesterday, 10:48 AM
0 responses
17 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...