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Hi,
Could somebody give me advices about the good practices to set the option "-D" of the vcfutils.pl varFilter script (from samtools/bcftools)? I have read that the option is useful to remove false positive SNPs due tu alignement artifacts, and that it must be set in function of the coverage you have in your data. If I have a mean coverage of 40X, how should I preferably set -D? -D80, -D120, ...?
Thank you for your help !
C.
Could somebody give me advices about the good practices to set the option "-D" of the vcfutils.pl varFilter script (from samtools/bcftools)? I have read that the option is useful to remove false positive SNPs due tu alignement artifacts, and that it must be set in function of the coverage you have in your data. If I have a mean coverage of 40X, how should I preferably set -D? -D80, -D120, ...?
Thank you for your help !
C.