Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Post here a screenshot of the alignments (tablet) for one of the snps and then we can discuss. The original SAM alignments for the SNP would help also.
-
thanks
Originally posted by drio View PostIf you have a very small number of SNPs, validate them by eye. Display the alignments at the SNP position and see if the call makes sense. Ultimately of course validate via sanger.
Leave a comment:
-
If you have a very small number of SNPs, validate them by eye. Display the alignments at the SNP position and see if the call makes sense. Ultimately of course validate via sanger.
Leave a comment:
-
Hi,
if you encounter an interesting SNP in your analysis, the quickest way to determine if it's real or not would be to confirm it with an independant method like PCR + Sanger ...
Leave a comment:
-
It could be a heterozygous SNP. Also errors can/will always occur (in sequencing, mapping), you might want to look at quality values as well.
Leave a comment:
-
how to determine a snp ?
hi, i resequenced a mutated bacteria genome, and want to detect the mutation information. at a position, i found half of the reads mapped are different from the reference, and half are the same . so some software reported it as snp, and some not. how to determine it as a SNP. how did this happened.
thanks.Tags: None
Latest Articles
Collapse
-
by seqadmin
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
Channel: Articles
07-08-2024, 03:19 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 06:46 AM
|
0 responses
9 views
0 likes
|
Last Post
by seqadmin
Yesterday, 06:46 AM
|
||
Started by seqadmin, 07-24-2024, 11:09 AM
|
0 responses
24 views
0 likes
|
Last Post
by seqadmin
07-24-2024, 11:09 AM
|
||
Started by seqadmin, 07-19-2024, 07:20 AM
|
0 responses
159 views
0 likes
|
Last Post
by seqadmin
07-19-2024, 07:20 AM
|
||
Started by seqadmin, 07-16-2024, 05:49 AM
|
0 responses
127 views
0 likes
|
Last Post
by seqadmin
07-16-2024, 05:49 AM
|
Leave a comment: