PL high support for each GT for all SNPS

htetre

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Join Date: Jul 2013

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#1

PL high support for each GT for all SNPS

08-15-2018, 10:01 AM

Hello,

I have two populations I am trying to discover SNPs for. In my .vcf file one population has the GT and the PL is 0,0,0 for every single snp ie 0/1, 0/0, and 1/1. Does anyone know where I went wrong? My sam to bam conversion shrunk my data file considerably (179G to 27G) compared to the second pooled population (173G to 46G) in this study. Other than that I cant seem to pinpoint a difference in my two samples.

Thank you for any and all help
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SNPsaurus

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Join Date: May 2013

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#2

08-16-2018, 08:55 PM

I haven't seen that error before. Were both bam files sorted? Can you make a vcf file for each sample?

Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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htetre

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#3

08-20-2018, 11:05 AM

I am currently trying to call SNPs with the problematic file alone. I am able to get expected results, ie high support for one GT and low support for the other two GT with the "non-problematic" file.
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