Sure Select Exon Capture

[manisisland]

Hey Guys,

For Agilent Sure Select All Exon kit, what is your experience with hybridization? The protocol suggests 24 to 72 hr. What is the standard practice now?

Thanks,
Manisisland

[ntremblay]

We have always used 24 hrs hybridization and always obtain great results ... also minimizing the hybridization time reduce the evaporation risk and the total hands-on time required.

The only time I've used the 72 hrs is when I've prepared libraries on a friday and left them for the week-end!

[manisisland]

Thanks ntremblay!
By the way, what is your on target rate?

[ntremblay]

Our on target rate is about 75% within 100 bp ...