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454 16S data from QIIME - what next?
Hi, totally new to this area, I've got some 454 16S data run through QIIME by another group, for diversity profiling. Data has been demultiplexed + assigned to taxomomic identities using GreenGenes 4/2/2011. I am installing QIIME on my computer so that I can get a better feel for how it works, but I wondered if anyone could point me in the direction of other tools I can use with this data in the meantime. Mainly I'd like to give some identity to all the bacteria that currently fall under "Other" and to produce some nice plots. Thanks for any help.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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