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  • jtotheulie
    Member
    • May 2016
    • 12
    #1

    Extraction

    Hi,

    My lab started doing metagenomic assays.
    We have ran into a problem.
    Our extractions are going great. The only downfall is that it elutes in a high volume, 200ul.
    We then desalt in a spin column, we lose half of our DNA.

    What is the best way to desalt to concentrate our samples?

    J
    Tags: None
    • thermophile
      Senior Member
      • Apr 2015
      • 243
      #2
      I had good luck with amicon columns for desalting and concentrating DNA for metagenomics. They are expensive though.
      Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.

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