Good evening,
I'm using MG-RAST for taxonomic separation of my sequences through the generation of the table, I select the filters I want and now my doubt is from what I should choose% so that the identity of my sequences does not by repeated, I mean, selecting only from 80%? 90%? to 100%? what the minimum percentage that can cover all the sequences, but without repeating them in two different groups, for example, the same sequence as being from fungus and as being from plant.
I apologize, I did not know if I could make myself clear ...
I'm using MG-RAST for taxonomic separation of my sequences through the generation of the table, I select the filters I want and now my doubt is from what I should choose% so that the identity of my sequences does not by repeated, I mean, selecting only from 80%? 90%? to 100%? what the minimum percentage that can cover all the sequences, but without repeating them in two different groups, for example, the same sequence as being from fungus and as being from plant.
I apologize, I did not know if I could make myself clear ...