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  • biofreak
    Member
    • Jun 2011
    • 44

    #1

    pre-built indexes

    Dear all,
    Where can I download bow-tie pre-built indexes for dog (canine) genome?
    thanks
  • dariober
    Senior Member
    • May 2010
    • 311

    #2
    If you can't find pre-built indexes make your own. Assuming you are happy with the ENSEMBL genome for dog, it should be just a matter of running:
    Code:
    cd path/to/my/bowtie/indexes
    ## Download ref genome
    wget ftp://ftp.ensembl.org/pub/release-63/fasta/canis_familiaris/dna/Canis_familiaris.BROADD2.63.dna.toplevel.fa.gz
    ## Create indexes
    bowtie-build Canis_familiaris.BROADD2.63.dna.toplevel.fa.gz Canis_familiaris.BROADD2.63.dna.toplevel.fa.gz
    Hope this helps
    Dario

    Comment

    • JQL
      Member
      • Apr 2011
      • 83

      #3
      I thought they have canine pre-built index file?

      http://bowtie-bio.sourceforge.net/index.shtml

      Comment

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