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  • cdb3
    Junior Member
    • Aug 2011
    • 4

    Convert SOAPsplice v1.4 output to SAM

    Hi all, I'm trying to use SOAPsplice 1.4 to align some paired end reads. The alignment works fine, but the output is a custom format. Does anyone know how to convert the SOAPsplice ouput to SAM or have a script to do this? Thanks!

    Note: I realize v1.4 of SOAPsplice "supports" output to SAM, but only from the soapsplice executable. However, the example included with the SOAPsplice download makes use of a few more scripts after the soapsplice executable, making the SAM output useless (not to mention I'm getting an error when I try to generate a SAM file from the soapsplice executable).

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
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    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

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