Recently I notice that the new version of Tophat does not output the read pairs which map to two different chromosomes. However, these alignments are outputted by old version. Does anyone know an option to get them out from the new Tophat?
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That is a bit scary. I wonder if this is a design shift with the introduction of tophat-fusion which is design for this issue. More I'm worried that a wierd cufflinks abundance issue with more "FAIL" genes might be related to a tophat error not cufflinks. Time for a matrix comparison of previous and current tophat version with previous and current cufflinks versions.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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