Hi everybody!
I'm a beginner in this subject and I have a doubt about the numbers of reads / coverage necessary for plant mRNA sequencing. I would like to sequence mRNA from apical meristems of a tree inoculated and non-inoculated with a pathogen, in order to identify the differential expression. No reference genome is available. I'm planning to perform 1x100 pb (illumina Hiseq) for each sample. According to the service provider they can guarantee 5-7.5 millions of reads per 10% channel. I wonder if it is enough for each sample. Where can I get some theoretical material about this issue? Thank you very much.
I'm a beginner in this subject and I have a doubt about the numbers of reads / coverage necessary for plant mRNA sequencing. I would like to sequence mRNA from apical meristems of a tree inoculated and non-inoculated with a pathogen, in order to identify the differential expression. No reference genome is available. I'm planning to perform 1x100 pb (illumina Hiseq) for each sample. According to the service provider they can guarantee 5-7.5 millions of reads per 10% channel. I wonder if it is enough for each sample. Where can I get some theoretical material about this issue? Thank you very much.
Comment