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  • MapSplice 1.15.2 error

    Dear SEQanswer community,

    I am running MapSplice 1.15.2 and get this error:

    [Wed Nov 16 11:38:23 2011] Preparing output location mapsplice_5171114w3/
    [Wed Nov 16 11:38:23 2011] Beginning Mapsplice run (v1.15.2)
    -----------------------------------------------
    bin directory: [~/bin/MapSplice_1.15.2/bin/]
    [Wed Nov 16 11:38:23 2011] Checking for chromosomes files or directory
    [Wed Nov 16 11:38:23 2011] Checking for chromosomes files or directory passed
    [Wed Nov 16 11:38:23 2011] Checking for Bowtie index files
    [Wed Nov 16 11:38:23 2011] check reads format
    [Wed Nov 16 11:42:10 2011] merge paired end reads remove short
    [Wed Nov 16 11:55:30 2011] Converting bowtie mapped to SAM format
    [Wed Nov 16 11:55:30 2011] divide reads
    [Wed Nov 16 12:21:32 2011] Mapping reads against btau_UMD31 with Bowtie
    [Wed Nov 16 13:18:13 2011] sort segmentbwt
    [Wed Nov 16 14:43:32 2011] reads all chromo sizes
    [Wed Nov 16 14:44:05 2011] mapsplice_search
    [Wed Nov 16 15:48:00 2011] Aligning spliced reads
    [Wed Nov 16 17:27:26 2011] mapsplice_report
    error: open reads file error
    [FAILED]
    Error: mapsplice_report failed

    I have searched for errors in the logfiles but everything seems to work properly.
    Does anybody know about this problem?

    Thanks in advance,
    hardi

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