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  • papori
    Senior Member
    • Dec 2010
    • 181

    #1

    directional mRNA sequencing on amplified RNA

    Hi,
    Do anyone know on paper of a directional mRNA(strand specific) sequencing on amplified RNA?
    Thanks
    pap
  • steven
    Senior Member
    • Aug 2009
    • 269

    #2
    Here is an interesting comparative study from Levin et al:
    Strand-specific, massively-parallel cDNA sequencing (RNA-Seq) is a powerful tool for novel transcript discovery, genome annotation, and expression profiling. Despite multiple published methods for strand-specific RNA-Seq, no consensus exists as to ...

    Comment

    • papori
      Senior Member
      • Dec 2010
      • 181

      #3
      Thanks steven,
      i am looking for paper that do amplification on low amount of RNA and then sequence it by strand specific..
      Do you know?

      i knew the paper that you mention, and its a really good one, but it isnt what i am looking for

      Comment

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