I tried to use tophat to generate input files for Altanalyzer
After downloading GRCh37.55.gtf from ensembl, I run
./tophat --GTF Homo_sapiens.GRCh37.55.gtf --output-dir /home/user/tophat_data/NP902 hg19 /home/user/hESC-NP/SRS011902-NP.fq
I got the error message:
Error: reading .... Homo_sapiens.GRCh37.55.gtf .
I tried to follow suggestions in this link http://seqanswers.com/forums/showthread.php?t=8247
But they do not work for my task.
Homo_sapiens.GRCh37.56.gtf and Homo_sapiens.GRCh37.65.gtf do not work either.
Any suggestions?
After downloading GRCh37.55.gtf from ensembl, I run
./tophat --GTF Homo_sapiens.GRCh37.55.gtf --output-dir /home/user/tophat_data/NP902 hg19 /home/user/hESC-NP/SRS011902-NP.fq
I got the error message:
Error: reading .... Homo_sapiens.GRCh37.55.gtf .
I tried to follow suggestions in this link http://seqanswers.com/forums/showthread.php?t=8247
But they do not work for my task.
Homo_sapiens.GRCh37.56.gtf and Homo_sapiens.GRCh37.65.gtf do not work either.
Any suggestions?
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