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  • lgarmire
    Junior Member
    • Nov 2008
    • 2

    #1

    RNASEQR can not generate anchor sequences

    Hi Guys,
    I wonder any of you started to look into the package RNASEQR that claims to be the best of all RNA-Seq packages (who knows!). http://nar.oxfordjournals.org/conten...r.gkr1248.full

    Does anyone have their old test sample set link? If so, I will appreciate the help. Basically, I used their test sample, but can not get any anchors generated while running the generate_anchors.py
    script (phase2.anchors.fastq file has no reads), although the
    phase2.sam input file looks fine.

    It looks like the awk command does not return anything:
    awk '^[^Q]/if { rshift($2, 2) %2==1) {print $1; print$10;$print11;}} phase2.sam
    neither the loop for line in ps1:....was called.

    What is this awk command trying to do?
  • AlbertOuTw
    Junior Member
    • Aug 2010
    • 6

    #2
    Dear Igarmire,

    There are some file format problem in the first released RNASEQR but they had modified those errors and have a new release in February. I tried the example data and it looks fine. Maybe you can try again.

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