Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • papori
    Senior Member
    • Dec 2010
    • 181

    #1

    tophat error

    Hi,

    when i am executing tophat like this:
    ./tophat -r 430 /mnt/FILE/zv9genome/zvgenome ../ex1/t_1 ../ex1/t_2

    i got this exception:

    [Sat Feb 4 18:00:11 2012] Beginning TopHat run (v1.4.0)
    -----------------------------------------------
    [Sat Feb 4 18:00:11 2012] Preparing output location ./tophat_out/
    [Sat Feb 4 18:00:11 2012] Checking for Bowtie index files
    [Sat Feb 4 18:00:11 2012] Checking for reference FASTA file
    [Sat Feb 4 18:00:11 2012] Checking for Bowtie
    Bowtie version: 0.12.7.0
    [Sat Feb 4 18:00:11 2012] Checking for Samtools
    Samtools Version: 0.1.18
    [Sat Feb 4 18:00:11 2012] Generating SAM header for /mnt/FILE/zv9genome/zvgenome
    format: fastq
    quality scale: phred33 (default)
    [Sat Feb 4 18:00:24 2012] Preparing reads
    left reads: min. length=101, count=5992
    right reads: min. length=101, count=5967
    [Sat Feb 4 18:00:24 2012] Mapping left_kept_reads against zvgenome with Bowtie

    gzip: stdout: Broken pipe
    [Sat Feb 4 18:00:27 2012] Processing bowtie hits

    Warning: junction database is empty!
    [Sat Feb 4 18:02:17 2012] Processing bowtie hits
    [FAILED]
    Error executing: /mnt/FILE/tophat-1.4.0.Linux_x86_64/bam_merge ./tophat_out/tmp/left_kept_reads.candidates_and_unspl.bam ./tophat_out/tmp/left_kept_reads.candidates.bam ./tophat_out/tmp/left_kept_reads.unspl.bam

    is the error result of the gzip broken pipe or from the junction database is empty?
    Do anyone know what is the gzip exception?

    Best,
    Pap

Latest Articles

Collapse

  • SEQadmin2
    Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
    by SEQadmin2



    CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

    Despite this, “CRISPR helped turn genome editing from a specialized technique into
    ...
    07-31-2026, 11:01 AM
  • SEQadmin2
    Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
    by SEQadmin2


    Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

    The systematic characterization of the human proteome has
    ...
    07-20-2026, 11:48 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 12:22 PM
0 responses
12 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 08-11-2026, 10:35 AM
0 responses
13 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 08-06-2026, 07:41 AM
0 responses
30 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 08-03-2026, 10:13 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Working...