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  • papori
    replied
    Originally posted by pmiguel View Post
    Could be that synthesis of the second strand ("turn around") went poorly for some reason. Or that the SBS reagents started to decline in capability as the days they spent at 4 oC increased.

    --
    Phillip
    thanks pmiguel !

    Leave a comment:


  • pmiguel
    replied
    Could be that synthesis of the second strand ("turn around") went poorly for some reason. Or that the SBS reagents started to decline in capability as the days they spent at 4 oC increased.

    --
    Phillip

    Leave a comment:


  • papori
    started a topic Paired End Seq

    Paired End Seq

    Hi,
    i just got rna data from illumina highseq2000.
    when i checked the data quality(FASTQC) i found that the first end has higher quality than the second end (by far...)

    Any idea why?

    Best,
    Pap

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