Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • R_user
    Junior Member
    • Mar 2012
    • 1

    #1

    EdgeR Normalization Question

    I am looking to do TMM normalization in an experiment where you have to do pairwise comparisons between 20+ samples in replicates of three (A1, A2, A3 vs B1, B2, B3; A1, A2, A3 vs C1, C2, C3; B1, B2, B3 vs C1,C2, C3 and so on).
    I was thinking that I should normalize all the samples together and then do pairwise comparisons.
    I was wondering how I can get EdgeR to give me the TMM normalized values in a csv file.
    Thanks!
  • Gordon Smyth
    Member
    • Apr 2011
    • 91

    #2
    There's actually no such thing as TMM normalized values. Normalization in edgeR is something that affects the fitted models rather than the data values. As the edgeR User's Guide says:

    "There are methods implemented in edgeR to normalize the counts for compositional bias in sequenced libraries and for diff erences between libraries in sequencing depth. These adjustments are off sets in the models used for testing DE and do not transform the counts in any way."

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 11:13 AM
    0 responses
    21 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, Yesterday, 10:48 AM
    0 responses
    18 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Working...