DNA conc. after PCR for small RNA Truseq?

[kia76]

Hi all, I am preparing libraries from total RNA using the most recent version of the small RNA Truseq kit (firs time user!!). I was wondering what DNA concentration I should expect after the PCR amplification step (starting from 1ug total RNA). We run the nanodrop before sending the libraries to the bioanalyzer and all our libraries seem to be in the 700-900 ng/ul range. Do these numbers make sense?
thanks!

[sjm]

Your concentrations seem to be quite high - I use the same kit, and have been achieving 10-20 ng/uL (in a 10 uL) volume from mouse and rat pancreatic tissue. I don't have my lab notebook in front of me but I think that's based on using 14 cycles of PCR amplification (I also begin with 1 ug total RNA).

Illumina do note in their manual that yields vary considerably between different tissue types. Also, if you've inadvertently gel-purified the incorrectly sized fraction at a slightly higher molecular weight, there's a lot more of it...