Hi there,
we're currently preparing RNA samples for small RNA Seq on the Illumina HiSeq using the new sample prep kit from NEB. For DNA digestion, usually EDTA is used to stop the DNase reaction. Do you know if that EDTA (at a conc of 8 mM) interferes with the library prep (adapter ligation, PCRs, etc.)?
Many thanks in advance!
we're currently preparing RNA samples for small RNA Seq on the Illumina HiSeq using the new sample prep kit from NEB. For DNA digestion, usually EDTA is used to stop the DNase reaction. Do you know if that EDTA (at a conc of 8 mM) interferes with the library prep (adapter ligation, PCRs, etc.)?
Many thanks in advance!
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