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  • vebaev
    Senior Res.
    • Oct 2008
    • 112

    #1

    Filtering Fastq after bcl-convertor CASAVA 1.8?

    Are fastq files need more filtering or they are ready to use/assemble/map since Casava 1.8 only passed seqs are remain in fastq?

    P.s
    My post i guess was for bioinfo tread sorry
    ------------
    SMART - bioinfo.uni-plovdiv.bg
  • HESmith
    Senior Member
    • Oct 2009
    • 512

    #2
    The chastity filter applies to the first 25 bases only. For most applications, it's a good idea to trim or mask adapters and low-quality bases as well.

    Comment

    • vebaev
      Senior Res.
      • Oct 2008
      • 112

      #3
      Thanks and what software I can use to do that in order to get at the end only the good reads which to use in the next analyses?
      ------------
      SMART - bioinfo.uni-plovdiv.bg

      Comment

      • HESmith
        Senior Member
        • Oct 2009
        • 512

        #4
        Trimmomatic is a useful tool for this application; others can be found in the wiki under 'trimming.'

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