Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    cuffdiff log2 value

    Hi,

    I am analyzing my RNA-Seq data. After running cuffdiff, I got a list of differentially expressed transcrpts or genes.

    As far as I know, log2 value = fold change. However, there are minus values. Is this possible?? log2 value can not be minus. Did I miss something??



    Looking forward to your help.

    Thanks in advance.

    Best
    Tags: None
  • #2
    The log2 value can most definitely be negative. Why not?

    Comment

    • #3
      Try log2(0.5) and log2(2). Log2 scale is convenient.
      /* Shawn Driscoll, Gene Expression Laboratory, Pfaff
      Salk Institute for Biological Studies, La Jolla, CA, USA */

      Comment

      Previous template Next

      Latest Articles

      Collapse
      • seqadmin
        Non-Coding RNA Research and Technologies
        by seqadmin


        Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
        ​
        [Article Coming Soon!]...
        Yesterday, 08:07 AM
      • seqadmin
        Recent Developments in Metagenomics
        by seqadmin





        Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...
        09-23-2024, 06:35 AM
      • seqadmin
        Understanding Genetic Influence on Infectious Disease
        by seqadmin




        During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.

        Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...
        09-09-2024, 10:59 AM
      View All

      ad_right_rmr

      Collapse

      News

      Collapse
      Topics Statistics Last Post
      Mechanical Forces in DNA Transcription Uncovered by Clemson Researchers by seqadmin
      Started by seqadmin, 10-02-2024, 04:51 AM
      0 responses
      14 views
      0 likes
      		 Last Post seqadmin
      by seqadmin
      10-02-2024, 04:51 AM  
      New Epigenetic Clock Links Cheek Cells to Mortality Risk by seqadmin
      Started by seqadmin, 10-01-2024, 07:10 AM
      0 responses
      25 views
      0 likes
      		 Last Post seqadmin
      by seqadmin
      10-01-2024, 07:10 AM  
      AI-Powered Blood Test Shows Promise for Early Ovarian Cancer Detection by seqadmin
      Started by seqadmin, 09-30-2024, 08:33 AM
      1 response
      31 views
      0 likes
      		 Last Post EmiTom
      by EmiTom
      Yesterday, 06:46 AM  
      Stem Cell Research Suggests Human Cells May Enter Developmental Pause by seqadmin
      Started by seqadmin, 09-26-2024, 12:57 PM
      0 responses
      20 views
      0 likes
      		 Last Post seqadmin
      by seqadmin
      09-26-2024, 12:57 PM  
      View All
      Working...
      X