RAW public SEq-data

[prussiap]

Hi folks,
I've been reading the forums to brush up on this stuff. I'm a geneticist. and want to get more into the bioinformatics side of things. Honestly I'm just looking for a simple RNA-seq fastq file. A raw unadulterated spit out of an illumina or solid machine.

I have looked at SRA and GEO and ArrayExpress.
It seems that those are already processed a bit, or are not labelled properly (I downloaded http://trace.ddbj.nig.ac.jp/DRASearch/run?acc=SRR389027) but it seems like it's all even 42bp long (which doesn't make sense as a paired end RNA-seq data). So it must have been cleaned.

any help with a link of a simple RNA-seq data would be greatly appreciated. I want to mess around with exome stuff alignment, etc just follow a dataset from start to end.

Thanks,

[alexdobin]

ENCODE RNA-seq

ENCODE generated a large RNA-seq dataset, multiple cell lines, A+/A-, nucleus/cytoplasm/whole cell, stranded dUTP protocols, 2 bio-reps. Raw .fastq files (as well as further processed files) are available from UCSC:

http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeCshlLongRnaSeq/

[prussiap]

perfect.. bingo.. thanks man!