Hi everybody!
I've got a RNA-Seq experiment which says:
"cDNA sequencing libraries were prepared using the Illumina mRNA sequencing prep kit"
I've to do alignment using TopHat and I don't known what to select for the --library-type option.
Here says that Standard Illumina protocol is unstranded, but I'm not sure...
Could someone confirm that?
Thanks!
I've got a RNA-Seq experiment which says:
"cDNA sequencing libraries were prepared using the Illumina mRNA sequencing prep kit"
I've to do alignment using TopHat and I don't known what to select for the --library-type option.
Here says that Standard Illumina protocol is unstranded, but I'm not sure...
Could someone confirm that?
Thanks!