Is it possible to control for or normalize RPKM data from two species in the same sample? I.e., if you have two cell types in a system where a drug treatment only kills one of the two cell types, is it possible to tell if the increased RPKM value in the cell type that isn't affected by the drug treatment is 'real' or simply a product of now having a higher relative number of total transcripts in the whole population.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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