Dear all,
I was going to do some simple t test on the FPKM values from Cufflink of two experimental conditions. However, I found one condition tend to have larger FPKM value than the other. I am wondering in practice, should the FPKM values be normalized for each sample? If so, how to normalize them?
Many thanks.
I was going to do some simple t test on the FPKM values from Cufflink of two experimental conditions. However, I found one condition tend to have larger FPKM value than the other. I am wondering in practice, should the FPKM values be normalized for each sample? If so, how to normalize them?
Many thanks.