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  • thejustpark
    Member
    • Jul 2012
    • 28

    #1

    Scripture doesn't recognize CIGAR string.

    Let me describe my problem with an example.

    This is my sorted, indexed sam file.
    seq.2851795 89 1 4470815 255 49M98N29M2S = 4470815 0 TGTGTGTGTGTGTGCGCGCGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTATGTGTGCG @@@DD@DDGDDFHGFHHGGIIEGGADBHIIIIIDEIIIHIIJGHEEGHGGHFEFDCC18/=/;;@)0&8>?@>B&25<98 OL:A:IH:i:1 HI:i:1

    When I run Scripture on this with appropriate size information and reference genome, I got this error.

    Using Version VPaperR3
    Has pairs: false
    Has upweighting turned on: false
    Computing weights..... upweighting? false weight: 1.0
    AlignmentDataModel loaded, initializing model stats
    Computing alignment global stats for chromosome 1
    model stats loaded, initializing model
    Built the model: 0.0010 free memory: 117707336
    Loaded chromosome Sequence
    Segmenting accross graph
    Going to get read iterator to make graph with counts
    Got read iterator
    Made it through all reads
    Collapsed reads
    Decollapsed by introns
    Made first graph
    Got extended pieces
    Exception in thread "main" java.lang.NullPointerException
    at broad.pda.seq.segmentation.ContinuousDataAlignmentModel.getNodeCounts(ContinuousDataAlignmentModel.java:1395)
    at broad.pda.seq.segmentation.ContinuousDataAlignmentModel.makeGraphWithCounts(ContinuousDataAlignmentModel.java:1359)
    at broad.pda.seq.segmentation.ContinuousDataAlignmentModel.contiguousAcrossGraph(ContinuousDataAlignmentModel.java:1192)
    at broad.pda.seq.segmentation.ContinuousDataAlignmentModel.main(ContinuousDataAlignmentModel.java:2209)

    But when I change the 5th column (the CIGAR string) into 80M, it works and returns a result.
    Scripture's web page says it takes in a `spliced read alignment', and my input indicates that it is a spliced read alignment. Then what's wrong in my file?

    FYI, I used both of the working version of Scripture (scripture-beta.jar and scripture-beta2.jar)
    Thanks in advance,
    HJ.

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