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  • abh
    Member
    • Aug 2012
    • 13

    #1

    demultiplexing fastq data(RNA-Seq data)

    Hi,

    I have 3 fastq files consisting of R1, R2 read files and
    a separate index file. for example They are in the format below.

    R1: @M00132:6:000000000-A0JG4:1:1:18014:1842 1:N:0:0
    Sequence data

    R2: @M00132:6:000000000-A0JG4:1:1:18014:1842 2:N:0:0
    Sequence data

    Index: @M00132:6:000000000-A0JG4:1:1:18014:1842 1:N:0:0
    CTCGGT
    +
    <@@DFD

    I want to split the R1 and R2 files based on this barcode.
    and create new files that contain these barcodes

    does any script or tool avaliable online so that i can work on it?
    Last edited by abh; 11-08-2012, 11:09 AM.

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