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  • DNA Sorcerer
    Member
    • Mar 2010
    • 24

    #1

    RNA-seq, fast turnaround needed

    I need to do a couple of transcriptomes but can't wait two months. Who is doing the job within a 4-week frame? -while keeping prices below outrageous of course-

    Thanks!
    Hi there
  • CarlHSLee
    Member
    • Aug 2012
    • 10

    #2
    Hi DNA Sorcerer, we can do it in your timeframe. Please contact me at [email protected]

    we are also located in Canada.

    RNA seq can be done using total RNA (we can prepare cDNA etc...)

    Thanks

    Carl

    Comment

    • Richard Barker
      Member
      • Apr 2012
      • 47

      #3
      I recormend the iPlant cyberinfrastruture for analysis. They have the Tuxedo2 protocol intergrated into a user friendly graphical interface that links to some supercomputer clusters in Arazona and Texas. This allows the analysis to be performed on the 'cloud' and therefore takes a fraction of the time compared to local computers and conventional university clusters. Its free and the almost limitless data store is itself pretty useful, so check out their website...

      Comment

      • DNA Sorcerer
        Member
        • Mar 2010
        • 24

        #4
        Thanks Richard, looks like a great resource, bookmarked.

        In this case I meant the actual sequencing service.
        Hi there

        Comment

        • Richard Barker
          Member
          • Apr 2012
          • 47

          #5
          Ooops, i see...
          I used the University of Wisconsin's biotechnilogy center, they had a fast turn around and produced high quality data...
          With rare exception, the single most important factor in determining the success of a single cell RNA-seq library preparation is the condition and health of the single cell/nuclei suspension at the time their transcripts are …

          Comment

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