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  • Xi Wang
    replied
    Sorry that yesterday our building encountered a power cut, so our web server was not at work. Our site works well now.

    Thanks.
    Xi


    Originally posted by svl View Post

    Leave a comment:


  • Xi Wang
    replied
    hi svl,

    Thanks for using DEGseq.

    If you want to install DEGseq from bioconductor, you should update your R to v2.10.0. Or you can install DEGseq by typing the following.

    --
    source("http://bioinfo.au.tsinghua.edu.cn/software/degseq/DEGseqInstall.R")
    --

    Best regards,
    Xi

    Originally posted by svl View Post
    I seem to be unable to install the package...anyone had succes?
    ----
    source("http://bioconductor.org/biocLite.R")
    biocLite("DEGseq")
    ----

    Also their site is unavailable: http://bioinfo.au.tsinghua.edu.cn/software/degseq
    Here is the page at Bioc: http://www.bioconductor.org/packages...ml/DEGseq.html

    Leave a comment:


  • svl
    replied
    Thanks. I figured it out. Had to update R and Bioconductor before trying to install the package....should keep my software better updated I guess

    Leave a comment:


  • RockChalkJayhawk
    replied
    Originally posted by svl View Post
    I seem to be unable to install the package...anyone had succes?
    ----
    source("http://bioconductor.org/biocLite.R")
    biocLite("DEGseq")
    ----

    Also their site is unavailable: http://bioinfo.au.tsinghua.edu.cn/software/degseq
    Here is the page at Bioc: http://www.bioconductor.org/packages...ml/DEGseq.html
    I had to try it a few times before I could get it loaded. Good luck.

    Leave a comment:


  • svl
    replied
    I seem to be unable to install the package...anyone had succes?
    ----
    source("http://bioconductor.org/biocLite.R")
    biocLite("DEGseq")
    ----

    Also their site is unavailable: http://bioinfo.au.tsinghua.edu.cn/software/degseq
    Here is the page at Bioc: http://www.bioconductor.org/packages...ml/DEGseq.html

    Leave a comment:


  • RockChalkJayhawk
    started a topic DEGseq

    DEGseq

    Hey all,

    A new package has come out for RNA Seq Analysis:

    DEGseq: an R package for identifying differentially expressed genes from RNA-seq data.
    Wang L, Feng Z, Wang X, Wang X, Zhang X.
    Bioinformatics. 2009 Oct 24. [Epub ahead of print]
    PMID: 19855105 [PubMed - as supplied by publisher]

    However, from my understanding (which may not be correct) they only use tags that map to the genome, not splice juntions. Does anyone else see this and if so, what would be the work-around?

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  • SEQadmin2
    Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
    by SEQadmin2



    CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

    Despite this, “CRISPR helped turn genome editing from a specialized technique into
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    07-31-2026, 11:01 AM

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