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  • seb330
    Junior Member
    • Oct 2012
    • 5

    #1

    qPCR to validate DGE

    I'm designing some qPCR reactions to test some candidate genes before we get our RNA-seq results back. Does anyone have a good suggestion for a control gene to use for bacterial samples? I've heard that just using 16S rRNA is problematic because it is so abundant. Also, since rRNA shouldn't be in my RNA-seq reactions, I'm hesitant to use that as a control. I've seen a lot of people use recA. Is this common, or are there other suggestions?
  • Pengfei Liu
    Member
    • Nov 2012
    • 16

    #2
    Do you have any idea on this issue now? I am also concerned with this problem.

    Comment

    • bernardo_bello
      Member
      • May 2012
      • 49

      #3
      Originally posted by Pengfei Liu View Post
      Do you have any idea on this issue now? I am also concerned with this problem.
      I'm also in search of bacterial qPCR standards.

      Bernardo

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