DESeq - Error: More than one fitInfo object available.

[tikacp]

hello,

i was trying out different methods to estimate the dispersions in my data and ran into the following problem for the 'per-condition' method ('blind' and 'pooled' work fine):

> cds <- newCountDataSet(exprs(dataNormGC),data$virtual_conditions)
> sizeFactors(cds) <- rep(1,18)
> cds <- estimateDispersions(cds,method="per-condition",sharingMode="maximum")
> plotDispEsts(cds)
Error in fitInfo(cds, name = name) : 
  More than one fitInfo object available. Specify by name. (See 'ls(cds@fitInfo)' for a list.)
Calls: plotDispEsts -> fitInfo

as suggested, i looked into

> ls(cds@fitInfo)

which only said

[1] "pooled"

can anyone explain to me why that happens?
in case it is important: my data contains replicates only for some conditions, not for all.

thanks
tim

[mdarby]

I am getting this same error. My data contains replicates for all conditions. Did you find an answer?

[tikacp]

hi,

unfortunately, i haven't found any solution, yet. i thought that maybe something attached to the workspace would cause the problem, but that wasn't it, either. i don't know where else to look currently, sorry.

cheers
tim