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  • aoifemc
    Junior Member
    • Aug 2012
    • 4

    Power in RNA Seq studies

    Hi,

    Does anyone have an ideas on how to go about doing power calculations for RNA Seq studies? I've seen this tool http://www.ncbi.nlm.nih.gov/pubmed/23314327 but I was wondering if anyone had other suggestions?. So far I've sequenced about 10 biological replicates in each of my groups that I'm comparing for DE but Im looking for a way of testing whether I need to do more samples or what kind of power I have with my data so far?

    Any advice would be appreciated!!
    thanks!

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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
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    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

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  • SEQadmin2
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