Hi every one
i work on RNA seq data. i used Tophat/cufflink pipeline to study referenced based RNA seq data analysis. after run all the step tophat, cufflink, and last cuffdiff for differential gene expression study i stuked at one place in my study. i want to extract differentially expressed gene/transcript sequence. but i dont know how can i extract gene/transcript sequence from my output files. i also have my reference genome file.how can i obtained this sequences can anybody help me to solve this problem? Thanks in advance.
i work on RNA seq data. i used Tophat/cufflink pipeline to study referenced based RNA seq data analysis. after run all the step tophat, cufflink, and last cuffdiff for differential gene expression study i stuked at one place in my study. i want to extract differentially expressed gene/transcript sequence. but i dont know how can i extract gene/transcript sequence from my output files. i also have my reference genome file.how can i obtained this sequences can anybody help me to solve this problem? Thanks in advance.
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