Calculate RPKM from BED file input, please help?

[luuloi]

Hi,

In some public RNA-seq data, it only available BED file as follow:

Assembly strand Start End readA readB id
1 - 4856258 4856285 ACACTCACACTACTAC... .... 2
...

Can I calculate the FPKM from this file as input? Please show me how? Any suggestion would be nice!

Thanks,
Loi

[rohitngs]

Where did you get the bed file from??
I want some source to download bed files for bacteria