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Hi,
To measure the expression level, two methods are commonly used, RNA-seq and tag-seq. The former is characterized by randomly break the cDNA and paired-end sequencing, while the latter only sequence the end or random of cDNA and single-end (50) sequencing. The major difference is the paired and single end sequencing. So the former can be used to do alternative splicing and other transcript structure analysis in addition to the expression level.
My question is, only for the capability of expression level measurement, are these two methods comparable? Any publications have studied this topic?
Thank you!
Best,
Pengcheng
To measure the expression level, two methods are commonly used, RNA-seq and tag-seq. The former is characterized by randomly break the cDNA and paired-end sequencing, while the latter only sequence the end or random of cDNA and single-end (50) sequencing. The major difference is the paired and single end sequencing. So the former can be used to do alternative splicing and other transcript structure analysis in addition to the expression level.
My question is, only for the capability of expression level measurement, are these two methods comparable? Any publications have studied this topic?
Thank you!
Best,
Pengcheng