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  • bhill
    Junior Member
    • Aug 2011
    • 5

    running Differential Expression on Cuffmerge tracking file

    My team is looking into running Differential Expression via GenePattern on the FPKM_tracking output from either Cuffmerge or Cuffcompare. Does anyone have experience with this or any warnings away from doing this, rather than using the FPKM_tracking output from cuffdiff?

    Thanks in advance
    Barbara Hill
    Software Test Engineer
    Cancer Informatics Development
    Broad Institute of Harvard and MIT
    Cambridge, MA
    USA

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
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