Hi
I get alot more DE genes when I analyse my RNA Seq dataset using the current version of DESeq versus DESeq2 -just using the default settings for both.
Its a simple design of 10 disease V 10 control samples but I get an extra ~1500 genes identified as significantly DE at an FDR of 5% using DESeq2.
I know DESeq2 has more powerful statistic etc but is this great of a difference likely or could I be doing something wrong...(more likely!) .
I'd appreciated any advice!
thanks!
I get alot more DE genes when I analyse my RNA Seq dataset using the current version of DESeq versus DESeq2 -just using the default settings for both.
Its a simple design of 10 disease V 10 control samples but I get an extra ~1500 genes identified as significantly DE at an FDR of 5% using DESeq2.
I know DESeq2 has more powerful statistic etc but is this great of a difference likely or could I be doing something wrong...(more likely!) .
I'd appreciated any advice!
thanks!
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