Help with low input RNA-seq experiment design
Hello,
Newbie here trying to move forward with an RNA-seq project. Thank you for your patience.
I have samples containing ~50ng of total RNA for RNA-seq. I am considering the SMARTER and OVATION technologies but have several questions regarding the workflow:
1) When (and how) should I remove rRNA? I suspect it needs to be done prior to reverse transcription, but the kits I found online require more starting material.
2) Do both kits support downstream barcoding for multiplexing?
3) Would it be possible to instead make cDNA and perform Whole Genome Amplification followed by KAPA kit?
Any other suggestions? I very much appreciate your feedback.
Thank you,
Ben
Newbie here trying to move forward with an RNA-seq project. Thank you for your patience.
I have samples containing ~50ng of total RNA for RNA-seq. I am considering the SMARTER and OVATION technologies but have several questions regarding the workflow:
1) When (and how) should I remove rRNA? I suspect it needs to be done prior to reverse transcription, but the kits I found online require more starting material.
2) Do both kits support downstream barcoding for multiplexing?
3) Would it be possible to instead make cDNA and perform Whole Genome Amplification followed by KAPA kit?
Any other suggestions? I very much appreciate your feedback.
Thank you,
Ben