Hello
I want to perform RNAseq on a low input sample. I would like to do RT-qPCR to verify that my samples are behaving as expected but unfortunately I will be using up almost all of my total RNA to generate a library (I have 110ng in my lowest sample input).
Is it possible to take some of the library and use it to do qPCR for my transcripts of interest?
Thank you
Ben
I want to perform RNAseq on a low input sample. I would like to do RT-qPCR to verify that my samples are behaving as expected but unfortunately I will be using up almost all of my total RNA to generate a library (I have 110ng in my lowest sample input).
Is it possible to take some of the library and use it to do qPCR for my transcripts of interest?
Thank you
Ben
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