Sequencing chemcially modified RNA bases

greg_hinkle

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Join Date: Jul 2010

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Sequencing chemcially modified RNA bases

08-09-2013, 09:05 AM

I would like to sequence a library of synthesized siRNAs.

Anyone have any experience sequencing chemically modified RNA on an Illumina platform? In particular 2' modifications like 2'-O-methyl or 2'-fluoro?

thanks
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