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  • BioInfoRx
    Junior Member
    • Dec 2009
    • 2

    Easy to Use Tissue-Specific Gene Expression Database

    The tissue-specific expression pattern can provide important clues about gene function. Here is a good online resource: Gene Atlas Database where you can search and plot gene expression data from RNA-Seq or other genomic data. The current database has Illumina Human Body Map data (RNA-Seq 16 tissues in human), and many array data sets (91 mouse tissues, 79 human tissues, NCI-60 cell lines, and human ES and iPS cells).

    Users can actually upload their own RNA-Seq data to create interactive online database use this system. If you are interested in testing this system with your data, please contact us at [email protected].

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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