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  • JonB
    Member
    • Jan 2010
    • 85
    #1

    Need advice: want to create a gtf file of transcript sequences

    Hi,

    I have a fasta file with 20000 transcript sequences. I want to create a gtf file containing the splicing information by mapping them to the genome. I tried using tophat2, but got an error message probably related to the length of the sequences. Any tips on how to make such file?

    Jon
    Tags: gtf
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